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循环中的microRNAs的跨冠状动脉度梯度

Salvatore De Rosa¹, Stephan Fichtlscherer, Ralf Lehmann

¹Division of Cardiology, Department of Medicine III, Goethe University Frankfurt, Frankfurt, Germany.

|October 5, 2011

概括

肌肉丰富的microRNAs (miRs),如miR-499和miR-133a,在心肌损伤时从心脏中释放出来. 这些miR与血管miR-126一起,显示了冠状动脉循环的差异调节,为心脏生物标志物提供了洞察力.

科学领域:

心血管生物学心血管生物学
分子心脏病学分子心脏病学
生物标志物发现发现

背景情况:

循环微RNAs (miRs) 被研究为心血管疾病的潜在生物标志物.
心脏对循环miRs的贡献需要阐明,特别是在心脏事件期间.

研究的目的:

为了确定心脏是否在心肌损伤期间释放特定的miRs进入循环.
在不同患者群体的冠状动脉循环中分析各种miRs的度梯度.

主要方法:

使用TaqMan聚合酶链反应在血中从大动脉和冠状动脉鼻样本中量化循环miRs.
在没有冠状动脉疾病,冠状动脉疾病稳定,以及急性冠状动脉综合征的患者中分析了miRs.

主要成果:

在急性冠状动脉综合征患者中,肌肉丰富的miRs (miR-499,miR-133a,miR-208a) 显著升高.
miR-499和miR-133a在冠状动脉循环中的水平增加,表明在心肌损伤期间释放.
miR-499梯度与心肌损伤程度相关 (高灵敏度的热素T).
血管miR-126水平在冠状动脉循环中下降,这表明消耗.

结论:

在心肌损伤期间,心脏会将肌肉丰富的miR-499和miR-133a释放到冠状动脉循环中.
血管 miR-126 在心肌损伤的跨冠状动脉通道中被消耗.
循环 miRs 的差调节为开发新型心脏生物标志物提供了潜力.

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