一个候选蛋白激酶C基因,PKC1,是S. cerevisiae细胞周期所需的
D E Levin1, F O Fields, R Kunisawa
1Department of Microbiology and Immunology, University of California, San Francisco 94143.
Cell
|July 27, 1990
概括
发芽的酵母Saccharomyces cerevisiae具有一个基因,PKC1,对细胞分裂至关重要. 这种基因的删除会导致细胞循环停止后DNA复制,表明由PKC1.1调节的新细胞循环检查点.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 蛋白激酶C (PKC) 异酶是哺乳动物细胞中的关键调节剂.
- 在酵母等较低的真核生物中,PKC同类的功能尚不清楚.
研究的目的:
- 识别和描述与哺乳动物蛋白激酶C相关的Saccharomyces cerevisiae中的基因.
- 研究已识别的酵母PKC基因在细胞循环调节中的作用.
主要方法:
- 使用cDNA探针对大鼠PKC异酶进行Saccharomyces cerevisiae基因组的选.
- 基因删除和枯竭研究,以分析PKC1损失的表型影响.
- 对缺乏PKC1的细胞进行细胞周期分析.
主要成果:
- 单个酵母基因PKC1,同类于哺乳动物PKCα,β和gamma亚种被分离出来.
- 删除PKC1导致了衰退性致死性.
- 缺少PKC1的细胞表现出细胞分裂周期 (cdc) 突变表型,阻止DNA复制后和小芽线分裂前的复制,这表明了一个新的检查点.
结论:
- 在Saccharomyces cerevisiae中的PKC1基因产物对细胞活力至关重要,并调节以前未知的细胞周期检查点.
- 这一发现表明,PKC信号通路在细胞周期控制中在真核生物中起着保留作用.
相关概念视频
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...