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Updated: May 28, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

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"火体":基于火的抗体探针,显示抗原依赖的光

Ryoji Abe1, Hiroyuki Ohashi, Issei Iijima

  • 1School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan.

Journal of the American Chemical Society
|October 8, 2011
PubMed
概括

我们开发了一种新的光生物传感器,通过测量光增强来检测抗原. 这种无试剂的"quenchbody"生物传感器为生物样本中的各种目标,包括药物和蛋白质,提供了高灵敏度.

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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