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相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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相关实验视频

Updated: May 28, 2026

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

通过可编程DNA链接器位移的快速多目标免疫磁分离.

Christine E Probst1, Pavel Zrazhevskiy, Xiaohu Gao

  • 1Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

Journal of the American Chemical Society
|October 13, 2011
PubMed
概括

这项研究引入了新的免疫磁性分离技术,用于多个生物分子和细胞的高通量隔离. 该方法利用DNA链接器来实现选择性移位,克服了传统单目标磁性测试的局限性.

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 免疫磁性分离对于隔离生物分子和细胞至关重要.
  • 目前的方法仅限于单个目标或单个参数研究.
  • 需要多目标分离技术.

研究的目的:

  • 开发一种先进的免疫磁性分离技术.
  • 为了使多个目标能够以高产量和纯度进行高通量分类.
  • 为了克服传统的"黑白"磁性测试的局限性.

主要方法:

  • 开发一种新的免疫磁性分离技术.
  • 使用可选择性取代的DNA链接器进行目标分类.
  • 从异质样本中进行多目标隔离的证明.

主要成果:

  • 使用新技术成功隔离了多个目标.
  • 在多目标分离中获得高产量和纯度.
  • 证明了DNA链接器的选择性移位能力.

结论:

  • 开发的免疫磁性分离技术可实现高效的多目标隔离.

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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

  • 这一创新扩大了磁性选择的应用范围,超出了单参数研究的范围.
  • 该技术有可能用于高通量生物样本的制备和分析.