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Updated: May 28, 2026

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
13:58

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Published on: September 26, 2011

使用在旋转式SlipChip上使用多体数RT-PCR测试艾滋病毒和C型肝炎病毒负载的旋转式SlipChip测试,对具有大动态范围的核酸进行多重量化量化

Feng Shen1, Bing Sun, Jason E Kreutz

  • 1Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States.

Journal of the American Chemical Society
|October 15, 2011
PubMed
概括

这项研究引入了新的微流体SlipChips,用于在资源有限的环境中准确量化HIV和HCV的病毒载量. 这些设备能够进行精确的数字RT-PCR测量,这对于有效的治疗监测和预防耐药性至关重要.

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...