霍克斯基因集群的动态架构
Daan Noordermeer1, Marion Leleu, Erik Splinter
1National Research Centre Frontiers in Genetics, School of Life Sciences, Ecole Polytechnique Fédérale (EPFL), Lausanne, Switzerland.
Science (New York, N.Y.)
|October 15, 2011
概括
在转录过程中,Hox基因集群的空间组织发生了变化. 不活跃的基因形成一个结构,而活跃的基因形成一个单独的隔间,揭示了脊椎动物身体轴模式的关键见解.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 对霍克斯基因转录的空间和时间控制对于脊椎动物的身体轴模式至关重要.
- 质子后翻译性修改涉及,但Hox集群的体内3D架构尚未得到充分理解.
研究的目的:
- 在转录激活过程中调查霍克斯基因集群的体内三维 (3D) 空间配置.
- 为了将3D架构的变化与染色质标记和基因活性相关联.
主要方法:
- 采用了高分辨率染色质构成捕获 (3C) 方法.
- 在胚胎小鼠组织中检查了霍克斯集群的空间配置,具有不同的霍克斯基因活性.
主要成果:
- 转录不活跃的霍克斯集群形成了一个单一的,独特的3D结构.
- 在转录启动时,Hox集群采用双模3D组织.
- 新激活的基因逐渐聚集到一个转录活跃的区间.
- 这种空间过渡与染色体标记的动态变化相关,这表明了转录状态.
结论:
- 霍克斯基因集群表现出与转录状态相关的动态3D组织变化.
- 霍克斯基因群的空间分区可能对这些基因群的直线激活至关重要.
- 这些发现为3D基因组架构在发育基因调节中的作用提供了体内证据.
相关概念视频
02:33Combinatorial Gene Control
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
02:07Organization of Genes
Overview
02:07Organization of Genes
Overview
01:30Structure of a Gene
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...