核糖体蛋白基因中的内子调节酵母核糖体的产生和功能
Julie Parenteau1, Mathieu Durand, Geneviève Morin
1Laboratoire de génomique fonctionnelle de l'Université de Sherbrooke, Département de microbiologie et d'infectiologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Québec, Canada.
Cell
|October 18, 2011
概括
酵母中的核糖体蛋白 (RP) 基因内子的分离对于细胞健康和应激反应至关重要. 重复的RP基因中的内子相互调节,影响核糖体的产生和功能.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 基因规则 基因规则
背景情况:
- 核糖体蛋白质 (RPs) 是核糖体生物发生过程中必不可少的.
- 前信使RNA (前mRNA) 的剪接是基因表达的一个关键步骤.
- 发芽酵母中的RP基因是高度拼接的,对细胞功能至关重要.
研究的目的:
- 调查酵母酵母中的核糖体蛋白 (RP) 基因中的内子的作用.
- 为了确定内部去除对RNA表达,rRNA前处理,细胞生长和应激反应的影响.
- 探索通过剪接介导的重复RP基因之间的调节关系.
主要方法:
- 在芽酵母中,从所有RP基因中系统地删除内部基因.
- 分析RNA表达,rRNA前处理和细胞生长表型.
- 评估细胞对各种压力条件的反应.
- 在重复的RP基因对中检查基因表达模式.
主要成果:
- 大多数RP基因内子对于最佳的细胞适应性和压力下生长至关重要.
- 在复制的RP基因的一个副本中的intron删除通常会非互惠地影响另一个副本的表达.
- 大约70%的重复RP基因表现出不对称的表达.
- 内子和基因删除表明了复制特异的表型效应.
结论:
- 酵母RP基因中的内子剪接在基因间调节中起着重要作用.
- 被内子影响的重复RP基因的表达比率与调节核糖体功能有关.
- 在酵母中,以内为媒介的调节对于维持细胞平衡和应激适应至关重要.
相关概念视频
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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
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Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
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