一个新的酶的进化为碳二硫化物转化由一个酸热友的archaeonon
Marjan J Smeulders1, Thomas R M Barends, Arjan Pol
1Department of Microbiology, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
Nature
|October 21, 2011
概括
研究人员在热友阿基亚中发现了一种新型酶,该酶可以代谢二硫化碳 (CS2). 这种酶是一种CS2酶,从古代的β-碳酸无水酶进化,在极端动物中显示出不同的进化.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 极端动物生物学 极端动物生物学
背景情况:
- 极端友好生物拥有独特的酶,用于专门的代谢途径.
- 在火山索尔法塔拉中,热爱酸的热友性古生物利用像H2S和CS2这样的减少硫化合物来获取能量.
- 这些硫化合物的氧化产生了硫酸的特征高度酸性条件.
研究的目的:
- 阐明碳二硫化物 (CS2) 酸酶的结构,机制和进化起源,该酸酶来自超热友的Acidianus菌株A1-3.3.
- 了解这种酶是如何进化而在极端环境中专门处理CS2的.
主要方法:
- 对CS2酶的结构分析.
- 生物化学测试以确定酶活性和基质特异性.
- 酶及其基因的比较基因组学和进化分析.
主要成果:
- CS2酶单体表现出β-碳酸无水酶折叠,但缺乏CO2活性.
- 一个不寻常的六角形连锁结构与大型终端臂形成一个独特的疏水道.
- 这条道作为一种特异性过器,使得疏水性CS2的选择性结合和水解成为可能.
- 基因周围的转子子序列表明,水平基因转移促进了它的获取.
结论:
- CS2酶代表了古代β-碳酸无水酶的分离进化.
- 它的独特结构,特别是疏水道,决定了CS2的基质特异性.
- 横向基因转移在极端动物中对CS2代谢的关键酶的进化中发挥了作用.
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