对低氧的平静反应受植物N端规则通路的调节
Daniel J Gibbs1, Seung Cho Lee, Nurulhikma Md Isa
1Division of Plant and Crop Sciences, School of Biosciences and Centre for Plant Integrative Biology, University of Nottingham, Loughborough LE12 5RD, UK.
Nature
|October 25, 2011
概括
N-end规则通路在植物中充当关键的氧气传感器,调节关键的转录因子以控制缺氧. 这一发现揭示了植物在低氧条件下生存的新型分子机制.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 植物需要氧气通过线粒体呼吸产生能量.
- 低氧 (低氧) 引发了植物的适应性代谢和转录变化.
- 与动物不同,植物缺乏一个明确的氧气感应机制来调节基因.
研究的目的:
- 调查N端规则路径在植物氧气传感中的作用.
- 在缺氧下识别N端规则路径的分子标.
- 阐明植物中低氧耐受性的机制.
主要方法:
- 对缺少N端规则通路组件的阿拉比多普西斯突变物进行遗传分析.
- 对低氧反应基因的基因表达分析.
- 在不同氧度下对转录因子进行蛋白质稳定性测试.
- 与大米 SUB1A-1.1 的比较分析.
主要成果:
- 缺乏N端规则通路组件的阿拉比多普西斯突变体构成性地表达缺氧反应基因,并表现出增强的缺氧耐受性.
- N-end规则路径针对含有Met-Cys N-终端基因的乙烯反应因子组VII转录因子 (例如,HRE2).
- 在低氧气下稳定HRE2可以改善植物的生存.
- 米SUB1A-1,尽管有图案,但不受N端规则路径的调节.
结论:
- N-end规则通路作为Arabidopsis中的关键氧气传感器,调节低氧反应转录因子.
- 这一途径提供了一种分子机制,用于感知和响应植物中严重的低氧水平.
- 米SUB1A-1从这种途径中脱离,表明替代机制有助于米品种的沉浸耐受性.
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