Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A minimal chemo-mechanical Markov model for rotary catalysis of F<sub>1</sub>-ATPase.

Nature communications·2026
Same author

Catestatin restores cardiac metabolic flexibility and enhances mitochondrial function.

Communications biology·2026
Same author

Hierarchical multi-timescale structural dynamics of the disordered N-terminal of p53.

Nature communications·2026
Same author

Diverse mechanisms of translation arrest by a Clostridia ribosome stalling peptide CliM.

Nature communications·2026
Same author

RNA promotes synapsin phase separation providing a platform for local translation.

bioRxiv : the preprint server for biology·2026
Same author

Bimodal Peptide Collision Cross Section Distribution Reflects Two Stable Conformations in the Gas Phase.

Journal of proteome research·2026

相关实验视频

Updated: May 8, 2026

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

通过离子蛋白质-脂质相互作用对膜蛋白质进行隔离.

Geert van den Bogaart1, Karsten Meyenberg, H Jelger Risselada

  • 1Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Nature
|October 25, 2011
PubMed
概括

对于神经元外细胞形成至关重要的合成素-1A聚类是由与酸-4,5-双酸盐 (PIP2) 的静电相互作用驱动的. 这种相互作用形成了独特的膜微域,对于突触囊泡释放至关重要.

更多相关视频

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
11:59

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
06:12

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Published on: January 27, 2021

相关实验视频

Last Updated: May 8, 2026

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
09:41

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
11:59

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
06:12

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Published on: January 27, 2021

科学领域:

  • 细胞生物学 细胞生物学
  • 神经科学是一个神经科学.
  • 生物化学 生物化学

背景情况:

  • 神经元外细胞分裂,即突触囊泡释放的过程,是由像syntaxin-1A.这样的蛋白质调节的.
  • 合成素-1A在外细胞分裂部位的等离子膜中聚集,但其分离的机制仍然不清楚.

研究的目的:

  • 阐明在血膜中合成素-1A封存和聚类背后的机制.
  • 研究特定脂质在合成素-1A微域形成中的作用.

主要方法:

  • 在PC12细胞中超分辨率刺激发射枯竭 (STED) 显微镜.
  • 涉及脂质操纵和蛋白质复制的生物化学测试.
  • 在人造膜系统 (巨型单状囊泡) 中分析脂蛋白相互作用.

主要成果:

  • 氨酸-4,5-双酸盐 (PIP2) 被确定为在合成素-1A丰富的微域中占主导地位的阴性脂质.
  • 对于合成素-1A的聚类,PIP2的积累是必不可少的;它被synaptojanin-1降解减少了聚类.
  • 合成素-1A和PIP2分离成不同的域在复制的囊泡中,独立于胆固醇.

结论:

  • 合成素-1A和PIP2之间的静电相互作用驱动特定的等离子膜微域的形成.
  • 这些PIP2介导的微域对于合成素-1A的局部化和神经元外细胞形成中的功能至关重要.
  • 蛋白质-脂质静电相互作用可以独立于胆固醇或其他脂质相行为而形成膜域.