Sirt5是一种依赖NAD的蛋白质氨酸脱氨酸酶和脱氨酸酶
Jintang Du1, Yeyun Zhou, Xiaoyang Su
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
Science (New York, N.Y.)
|November 15, 2011
概括
Sirt5是一种哺乳动物的sirtuin,可以有效地从蛋白质中去除 succinyl 和 malonyl 组. 这种Sirt5的蛋白质氨酸修饰逆转表明了 in vivo 的新调节机制.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 翻译后修改 翻译后修改
背景情况:
- 锡尔图因 (静音信息调节剂2蛋白) 是NAD+依赖的酶,参与关键的生物过程.
- 哺乳动物拥有七种sirtuins (Sirt1-Sirt7),其中Sirt4-Sirt7表现出有限或没有脱乙酶活性.
研究的目的:
- 为了研究Sirt5除去乙化之外的酶活性.
- 识别和描述Sirt5在蛋白质氨酸糖化和糖化中的作用.
主要方法:
- 在体外酶分析以确定Sirt5的desuccinylase和demalonylase活性.
- 质谱测量用于识别哺乳动物中的化和化蛋白质.
- 对Sirt5淘汰赛小鼠的分析,以评估蛋白质化在体内对蛋白质化的影响.
主要成果:
- 在实验室中,Sirt5显示了有效的蛋白质氨酸脱氨酸酶和脱氨酸酶活性.
- 在Sirt5的基囊中,特定的残留物 (Arg105,Tyr102) 被确定为基质偏好的关键.
- 在Sirt5缺乏的小鼠中观察到碳基酸盐合成酶1的化增加.
结论:
- Sirt5作为一个主要的desuccinylase和demalonylase功能,逆转特定的翻译后修改.
- 蛋白质氨酸酸化是一种可逆的修饰,Sirt5在体内调节.
- 通过脱化,Sirt5在调节代谢途径方面发挥着重要作用.
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