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核结构之间的ncRNA和Pc2甲基化依赖的基因转移介于基因激活程序.
Liuqing Yang1, Chunru Lin, Wen Liu
1Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093-0648, USA.
Cell
|November 15, 2011
概括
聚二蛋白 (Pc2) 的甲基化通过非编码RNA (ncRNAs) 控制核结构之间的生长基因运动. 这将核架构与基因表达调节联系起来.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 细胞核具有与非编码RNA (ncRNA) 相关的结构.
- 这些结构在调节转录中的作用尚不清楚.
研究的目的:
- 研究核架构结构,ncRNA和转录调节之间的关系.
- 阐明增长控制基因在细胞核内转移的机制.
主要方法:
- 研究了Polycomb 2蛋白 (Pc2) 甲基化和脱甲基化的作用.
- 研究了Pc2与ncRNAs TUG1和MALAT1/NEAT2.2的结合.
- 研究了聚合体 (PcG) 和染色体间颗粒 (ICG) 之间基因转移的影响.
- 分析了对核心压缩机/协同激活器组件和基质子代码阅读器的影响.
- 评估了NEAT2-Pc2相互作用在E2F1SUMOylation和基因激活中的作用.
主要成果:
- Pc2甲基化状态决定了增长控制基因在PcG和ICG之间的转移.
- Pc2根据其甲基化状态与特定的ncRNA (PcG中的TUG1,ICG中的MALAT1/NEAT2) 结合.
- ncRNAs促进调节性蛋白质复合体的组装,并影响基因素标记的识别.
- NEAT2与非甲基化Pc2结合促进了E2F1SUMOylation,激活了生长基因.
结论:
- 一个分子通路将亚核结构特定的ncRNA和蛋白质甲基化与基因转移连接起来.
- 这一途径通过将核架构与转录控制联系起来来实现协调的基因表达程序.
- 这些发现揭示了通过核组织的动态变化调节生长控制基因的新机制.
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