广泛表达的HLH基因网络调节组织特异性的细胞命运
Abhishek Bhattacharya1, Nicholas E Baker
1Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Cell
|November 15, 2011
概括
一个涉及Daughterless (Da) 和Extramacrochaetae (Emc) 蛋白质的调节网络控制细胞分化. 这种保存机制平衡了分化与生长和生存,影响了螺旋环-螺旋环蛋白的发育调节.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学是一种遗传学.
- 分子生物学分子生物学
背景情况:
- 基本螺旋环螺旋 (bHLH) 转录因子对于细胞分化至关重要.
- bHLH异构体伙伴的表达显著影响差异化结果.
- 了解这些合作伙伴的监管是理解发展过程的关键.
研究的目的:
- 调查一个独特的监管机制,控制bHLH异构体合作伙伴的表达.
- 阐明无女儿 (Da) 和外巨 (Emc) 蛋白在虫发育中的作用.
- 为了确定这种调节机制是否在不同的组织和物种中得到保护.
主要方法:
- 在Drosophila.中对交叉相互作用的调节网络的分析.
- 研究涉及Emc和Da的转录反循环.
- 检查细胞外信号对Emc表达的影响.
- 在多个Drosophila组织和哺乳动物细胞中进行比较分析.
主要成果:
- 一个监管网络通过反循环将Da和Emc表达联系在一起.
- 广泛的Emc表达抑制了Da,反对分化,同时促进了生长和生存.
- 抑制Emc的细胞外信号导致Da表达的增加.
- 这项规定定义了不依赖于bHLH基因的proneural ectoderm区域.
结论:
- 涉及Da和Emc的保护性调节机制平衡了与生长和生存的差异化.
- 这个网络提供了一个定义发展领土的机制.
- 这些发现表明,螺旋环-螺旋环蛋白质的发展调节保持的一般特征.
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