小鼠和人类干细胞因子DNAs的主要结构和功能表达
F H Martin1, S V Suggs, K E Langley
1AMGEN Inc., AMGEN Center, Thousand Oaks, California 91320.
Cell
|October 5, 1990
概括
研究人员分离了大鼠和人类干细胞因子 (SCF) 克隆,表达了活跃的截断形式. SCF增强了髓状和淋巴状原体的增殖,显示了与殖民地刺激因素的协同作用,以增加细胞生长.
科学领域:
- 分子生物学分子生物学
- 血液学 血液学 血液学
- 细胞生物学 细胞生物学
背景情况:
- 干细胞因子 (SCF) 是一个关键的细胞因子,参与血液形成.
- 了解SCF的结构和功能对于造血干细胞研究至关重要.
研究的目的:
- 为了隔离和表征大鼠和人类干细胞因子 (SCF) cDNA和基因组克隆.
- 为了表达和验证截断的SCF蛋白的生物活性.
- 研究SCF在造血原生细胞增殖中的作用及其与其他生长因子的协同作用.
主要方法:
- 对老鼠和人类SCF的部分和全长cDNA和基因组克隆的分离.
- 截断的SCF蛋白在大肠杆菌和哺乳动物细胞中的表达.
- 在骨髓培养物中评估SCF对髓状和淋巴状造血原体扩散的影响.
- 评估SCF与殖民地刺激因素的协同效应.
主要成果:
- 成功分离了老鼠和人类SCF克隆.
- 在表达的截断的SCF蛋白中证明生物活性.
- 通过SCF增加髓状和淋巴状造血原体的扩散.
- SCF与殖民地刺激因素的强有力的协同活动,增加殖民地数量和大小.
结论:
- 鼠和人类的SCF基因已经被克隆和特征化.
- 表达的截断的SCF蛋白质具有生物活性.
- 脑脊髓细胞在造血原生细胞的增殖中起着重要作用,并与脑脊髓细胞表现出协同效应.
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