从戈尔吉到等离子体膜的散装流量速度
A Karrenbauer1, D Jeckel, W Just
1Institut für Biochemie I, Universität Heidelberg, Federal Republic of Germany.
Cell
|October 19, 1990
概括
研究人员合成了活细胞分泌的水溶性斯芬戈米林类似物. 这项研究追踪了这种类似物通过戈尔吉装置的运输,揭示了细胞脂质处理和运输通路的洞察力.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 脂质代谢 脂质代谢是什么
背景情况:
- 斯芬哥米林和甘油脂是必需的细胞脂质.
- 它们的合成和运输涉及戈尔吉装置.
- 了解这些过程是细胞功能的关键.
研究的目的:
- 为了合成水溶性类比的sphingomyelin.
- 研究其在活细胞中由戈尔吉装置的利用和运输.
- 为了确定其分泌的动力学,并推断ER-to-Golgi传输速率.
主要方法:
- 一个截断的C8C8陶胺类同类物的合成.
- 用中国仓鼠卵巢 (CHO) 细胞进行化.
- 在37°C和30°C测量模拟分泌率.
- 与ER光内标记物的分泌速率进行比较.
主要成果:
- 截断的胺类似物是水溶性的,但细胞透.
- 活细胞利用类似物产生和分泌水溶性截断脂和糖脂.
- 在37°C时,类似物分泌的半衰期约为10分钟.
- 在30°C时,模拟的分泌半衰期为~14分钟,这表明快速的ER到戈尔吉运输.
结论:
- 一种截断的斯芬戈米林类似物可以通过细胞机械进行处理和分泌.
- 戈尔吉仪器可以利用这种类似物产生截断的脂质.
- 从ER到Golgi的运输速度很快,半段时间大约为4分钟,一半的ER可以排出.
相关概念视频
Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
Introduction to Membrane Traffic
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Transport Across the Golgi
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Introduction to Membrane Traffic
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...


