相关实验视频
Updated: Jun 17, 2026

14:29
Examination of Thymic Positive and Negative Selection by Flow Cytometry
Published on: October 8, 2012
通过II类主要基因相容性复杂分子对TCR玛三角+T细胞进行异常选择
L Lefrancois1, R LeCorre, J Mayo
1Department of Cell Biology, Upjohn Company, Kalamazoo, Michigan 49001.
Cell
|October 19, 1990
概括
主体自身相容性复合体 (MHC) 抗原影响T细胞受体 (TCR) 马三角体在CD8+内皮细胞淋巴细胞 (IEL) 中的使用. 这表明这些细胞有一个外性选择机制.
科学领域:
- 免疫学 免疫学 免疫学
- T细胞生物学T细胞生物学
- 胃肠道免疫学 胃肠道免疫学
背景情况:
- CD8+内皮性淋巴细胞 (IELs) 是肠壁中的重要免疫细胞.
- T细胞受体 (TCR) 三角体的使用是多样化的,其调节尚未完全理解.
- MHC抗原在塑造IEL谱系中的作用是一个正在积极研究的领域.
研究的目的:
- 调查MHC抗原对TCR马三角体使用在CD8+IELs的影响.
- 确定MHC是否影响特定TCR V三角洲地区的选择.
- 探索CD8+IEL中TCR马三角选择的机制.
主要方法:
- 使用的单克隆抗体 (MAbs) 特定于TCR gamma delta的泛反应和V delta 4区域.
- 分析了来自各种MHC哈普洛类型的小鼠的IEL,包括F1和重组杂交菌株.
- 检查了在没有胸腺的仿真小鼠中的TCR马三角选择.
主要成果:
- 在具有H-2k MHC单体型的小鼠中观察到V delta 4+ CD8+ IEL的显著更高的百分比.
- 对F1和复合菌株的分析表明,MHCII类,特别是IE表达,是增加Vdelta4使用所需的.
- 在奇米鼠中,TCR gamma delta 选择独立于胸腺发生.
结论:
- 在CD8+IEL中,MHC抗原在TCR马三角体的异形选择中发挥着关键作用.
- 这些发现表明,对马三角 TCRs 有一种新的异形选择性机制.
- CD8+ IEL可能具有MHCII类受限制的抗原识别能力.
相关概念视频
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Diversity of Antigen Receptors
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Naive T cells that have not yet encountered an antigen express two primary CD...
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