通过glyoxal-peroxynitrite系统生成单片氧
Júlio Massari1, Rita Tokikawa, Danilo B Medinas
1Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
Journal of the American Chemical Society
|November 22, 2011
概括
这项研究揭示了glyoxal与过氧酸盐快速反应,产生酸盐离子和单片氧. 这些发现揭示了疾病中氧化和碳酸应激的分子机制.
科学领域:
- 生物化学 生物化学
- 化学生物学 化学生物学
- 自由基化学 自由基化学
背景情况:
- 甲基乙烯,甲基甘和甘等α-dicarbonyls是反应性代谢产物.
- 这些化合物可以添加蛋白质和核基,从而导致细胞损伤.
- 过氧酸盐是一种强大的生物氧化剂,参与了化和氧化应激.
研究的目的:
- 为了研究谷氨酸和过氧酸盐之间的反应机制.
- 为了识别反应过程中形成的反应中间体和产品.
- 探索反应性氧物种的生成,特别是单片氧.
主要方法:
- 电子偏磁共振 (EPR) 光谱检测激素中间体.
- 直接流体实验和旋转捕获技术.
- 在1270nm处检测单个氧气排放.
- 使用HPLC/MS/MS对单片氧进行同位素标记和化学捕获.
主要成果:
- 格里奥克萨尔与过氧酸盐反应,以高速度产生酸盐离子.
- 通过EPR检测到含水形式基和基添加物.
- 通过近红外辐射和化学捕获确认了单点氧气的产生.
- 单片氧气的形成与甲基基和随后的反应有关.
结论:
- 格里奥克萨尔与过氧酸盐的反应产生甲酸盐和单片氧.
- 这一途径有助于理解碳和化应激.
- 这些发现提供了对炎症和衰老中的分子事件的见解.
相关概念视频
Radical Autoxidation
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
Oxidative Cleavage of Alkenes: Ozonolysis
In ozonolysis, ozone is used to cleave a carbon–carbon double bond to form aldehydes and ketones, or carboxylic acids, depending on the work-up.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
Oxygenic Photosynthesis
Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate light...
Autoxidation of Ethers to Peroxides and Hydroperoxides
Ethers represent a class of chemical compounds that become more dangerous with prolonged storage because they tend to form explosive peroxides when standing in the air. Autoxidation is the spontaneous oxidation of a compound in air. In the presence of oxygen, ethers slowly oxidize to form hydroperoxides and dialkyl peroxides.
Oxidation of Alcohols
In this lesson, the oxidation of alcohols is discussed in depth. The various reagents used for oxidation of primary and secondary alcohols are detailed, and their mechanism of action is provided.
The process of oxidation in a chemical reaction is observed in any of the three forms:
The process of oxidation in a chemical reaction is observed in any of the three forms:
Oxygen Requirements and Growth Patterns
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...


