具有令人印象深刻的NIR发射的水溶性线粒体特异性伊特尔复合物
Tao Zhang1, Xunjin Zhu, Chopen C W Cheng
1Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR China.
Journal of the American Chemical Society
|November 23, 2011
概括
一种新型的水溶性伊特复合物 (Yb-2) 与罗达胺B具有线粒体特定的局部化和强大的两光子近红外 (NIR) 发射. 这一进步在生物成像中提供了有前途的应用,因为它在水溶液中具有高的NIR发射量子产量.
科学领域:
- 协调化学 协调化学
- 生物有机化学 生物有机化学
- 材料科学 材料科学 材料科学
背景情况:
- 开发用于生物应用的新型发光探针至关重要.
- 近红外 (NIR) 发射探测器为深层组织成像提供了优势.
- 针对线粒体的探针对于研究细胞过程非常有价值.
研究的目的:
- 合成和表征一种与罗达胺B (Yb-2) 结合的水溶性甲酸伊特复合物.
- 为了研究Yb-2复合体的亚细胞定位和光物理特性.
- 评估Yb-2作为一种用于生物成像的两光子诱导的NIR发射探针的潜力.
主要方法:
- 合成和Yb-2复合物的特征.
- 用于亚细胞局部化研究的共聚焦显微镜.
- 在水溶液和DMSO中进行光谱测量 (吸收,排放,量子产量).
- 两光子激发光谱学. 两个光子激发光谱.
主要成果:
- Yb-2复合体证明了线粒体特有的亚细胞局部化.
- 强烈的两光子诱导的NIR辐射在650nm (氨酸合物) 和1060nm (Yb (III) 过渡) 观察到.
- 该综合体在不同激发波长的水溶液中表现出令人印象深刻的Yb(III) NIR发射量子产率为1%和2.5%.
结论:
- Yb-2复合体是一种水溶性,向线粒体的探测器,具有高效的两光子NIR发射.
- 它的光物理特性,包括高量子产量,使其适用于先进的生物成像应用.
- 这项研究为深度细胞和组织成像提供了一个有希望的新工具.
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