发芽酵母中Hsp104相关蛋白质聚合物的运动性和分离
Chuankai Zhou1, Brian D Slaughter, Jay R Unruh
1Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.
Cell
|November 29, 2011
概括
酵母细胞以不对称的方式分离蛋白质聚合物. 从芽中的聚合物清除依赖于Hsp104p的伴侣,而不是formins,并且涉及随机运动和溶解.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 蛋白质聚合物在酵母细胞分裂过程中积聚.
- 受损蛋白质的不对称分离发生在母细胞和芽细胞之间.
- 假设基于actin电缆的逆行运输可以从芽中清除聚合物.
研究的目的:
- 为了研究酵母中热诱导和自然存在的蛋白质聚合物的动态.
- 为了确定福明蛋白和Hsp104p伴侣蛋白在聚合物清除中的作用.
- 模拟不对称蛋白质聚合物遗传背后的机制.
主要方法:
- 视频显微镜的使用方法
- 颗粒追踪分析的分析
- 图像相关性分析分析
- 数学建模的数学建模
主要成果:
- 蛋白质聚合物表现出无定向偏差的随机步行动态.
- 热诱导的聚合物从芽中的清除是通过Hsp104p伴侣依赖溶解,而不是formins.
- 老细胞中自然形成的聚合物也表现出随机行走,但不会溶解.
- 建模表明缓慢的扩散和细胞几何构造有助于不对称的分离.
结论:
- Hsp104p 伴奏子对于热诱导的蛋白质聚合物的溶解至关重要.
- 甲蛋白对于清除这些聚合物从酵母中来说并不必要.
- 蛋白质聚合物的不对称遗传可以通过扩散和细胞形状等物理因素来解释.
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