阿拉比多普西斯EDS1将病原体效应体识别与细胞区特异性免疫反应联系起来
Katharina Heidrich1, Lennart Wirthmueller, Céline Tasset
1Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions, D-50829 Cologne, Germany.
Science (New York, N.Y.)
|December 14, 2011
概括
像TIR-NB-LRRs这样的植物免疫受体使用增强的疾病易感性1 (EDS1) 来触发防御. EDS1将病原体识别与核和细胞质防御通路联系起来,协调免疫力.
科学领域:
- 植物免疫力和抗病能力.
- 分子植物病原体相互作用
- 植物中的细胞信号通路.
背景情况:
- 植物细胞内核酸结合-氨酸丰富重复 (NB-LRR) 受体检测病原体效应体.
- 在NB-LRR受体激活和下游植物防御反应之间的联系尚未完全理解.
- 增强的疾病易感性1 (EDS1) 对于托尔-互白素-1受体 (TIR) -NB-LRR介导的免疫非常重要.
研究的目的:
- 阐明EDS1将TIR-NB-LRR受体激活与植物防御通路连接的分子机制.
- 研究EDS1在整合不同细胞区的防御信号中的作用.
主要方法:
- 在活的烟草细胞和阿拉比多普西斯树叶提取物中检测RPS4-EDS1和AvrRps4-EDS1复合体.
- 烟草细胞中蛋白质的暂时联合表达.
- 对细菌效应因子AvrRps4在细胞质或细胞核强制定位后的防御反应的分析.
主要成果:
- 已经证明EDS1能够分子地将细菌效应因子AvrRps4的TIR-NB-LRR蛋白RPS4识别与下游防御通路联系起来.
- 在细胞核和细胞质中检测到RPS4-EDS1和AvrRps4-EDS1复合体,这表明它在细胞区间中的作用.
- 核防御途径限制了细菌的生长,而编程细胞死亡和转录强化需要核细胞质协调.
结论:
- EDS1作为激活的TIR-NB-LRR受体的关键信号转换器,将病原体识别与防御反应联系起来.
- EDS1集成了细胞核和细胞质之间的防御信号,强调了核-细胞质协调在植物免疫中的重要性.
- EDS1的功能既是效应器目标,也是传输免疫信号在细胞区间中的关键组成部分.
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