病原体效应者准Arabidopsis EDS1并改变其与免疫调节者的相互作用
Saikat Bhattacharjee1, Morgan K Halane, Sang Hee Kim
1Division of Plant Sciences, Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA.
概括
阿拉比多普西斯增强的疾病易感性1 (EDS1) 蛋白质复合体与耐药性蛋白质RPS4/RPS6和SRFR1. 细菌效应剂AvrRps4和HopA1破坏了这些复合体,揭示了效应剂触发和基础免疫之间的直接联系.
科学领域:
- 植物免疫力 植物免疫力
- 分子植物病原体相互作用
- 蜂信号传输是如何进行的
背景情况:
- 植物耐药性蛋白质在检测到病原体因子时启动效应器触发的免疫力.
- 已经确定了很少的下游免疫调节剂,没有一个是已知的效应因子毒性标.
研究的目的:
- 为了识别抵抗蛋白的下游免疫调节者.
- 调查增强疾病易感性1 (EDS1) 在植物免疫力中的作用.
- 了解病原体效应因子如何与植物免疫成分相互作用.
主要方法:
- 同免疫沉用于识别蛋白质复合体.
- 同焦显微镜可视化蛋白质定位.
- 在Arabidopsis.中细菌效应体表达.
主要成果:
- EDS1与Toll-interleukin-1受体-核酸结合-丰富白重复 (TIR-NB-LRR) 抵抗蛋白RPS4和RPS6以及在细胞质膜上的SRFR1形成蛋白质复合体.
- 细菌效应器 AvrRps4 和 HopA1 破坏了这些含有 EDS1 的复合体.
- EDS1在TIR-NB-LRR介导的免疫和基底抵抗信号之间起着至关重要的作用.
结论:
- EDS1与TIR-NB-LRR免疫的关联很可能是由于这些耐药性蛋白直接保护.
- 通过TIR-NB-LRR介导的效应器触发免疫的激活直接通过EDS1.1连接到基底抵抗路径.
- 病原体作用者向EDS1复合体以调节植物免疫力.
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