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相关概念视频

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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相关实验视频

Updated: May 26, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
14:43

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

帕拉介导的细胞表面标签

Christopher D Spicer1, Therese Triemer, Benjamin G Davis

  • 1Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Mansfield Road, Oxford OX1 3TA, UK.

Journal of the American Chemical Society
|December 20, 2011
PubMed
概括
此摘要是机器生成的。

苏子-米亚乌拉合使得使用非自然氨基酸在细菌细胞表面上形成良性C-C键. 这种位点选择性方法对复杂生物体中的体内应用有希望,因为它具有无毒的催化剂.

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Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

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Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
11:39

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

相关实验视频

Last Updated: May 26, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
14:43

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
11:39

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

科学领域:

  • 生物化学 生物化学
  • 合成生物学 合成生物学
  • 催化剂是一种催化剂.

背景情况:

  • 细胞表面工程对于各种生物应用至关重要.
  • 开发选择性和生物相容的化学修饰方法是必不可少的.

研究的目的:

  • 为了在细胞表面实现选择性C-C键的形成.
  • 探索在催化中使用基因组合的非自然氨基酸.
  • 评估催化系统对于潜在的体内应用的生物相容性.

主要方法:

  • 在基因上将非天然的氨基酸与化侧链结合到大肠杆菌中.
  • 使用苏子-米亚乌拉合与无非催化剂的C-C键形成.
  • 在需要的负载下评估催化后的细胞活力.

主要成果:

  • 在大肠杆菌细胞表面的基因定义位置成功形成良性C-C键.
  • 没有素的催化剂在有效的度下没有显著的细胞死亡.
  • 在不损害细胞活性的情况下,实现了选择性细胞表面操纵.

结论:

  • 不自然氨基酸的苏苏基-米亚乌拉合提供了一个强大的方法,用于特定站点的细胞表面功能化.
  • 开发的催化系统是生物相容的,为体内应用铺平了道路.
  • 这种方法有可能在未来更复杂的生物体中形成催化金属介导的键.