区域特定的等离子组件用于现场活细胞拉曼光谱
Liguang Xu1, Hua Kuang, Chuanlai Xu
1School of Food Science and Technology, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214122, People's Republic of China.
Journal of the American Chemical Society
|December 24, 2011
概括
研究人员开发了一种新方法,使用DNA修饰的纳米粒子和纳米棒创建稳定,复杂的等离子组件. 这些组件可以作为细胞内探针用于实时监测细胞环境.
科学领域:
- 纳米技术 纳米技术
- 塑制剂的使用方法
- 生物物理学的生物物理.
背景情况:
- 等离子组装属性取决于几何组织.
- 来自纳米粒子 (NP) 的复杂等离子体超结构已得到充分研究,但来自不同构件的可重复组件的理解较少.
- 多样化的等离子组合为基础科学和光学应用中区域特定的异构体提供了潜力.
研究的目的:
- 开发一种合成方法,从几何多样化的构建块 (NP 和纳米棒,NR) 制造稳定且结构可重复的等离子组件.
- 研究这些组件作为细胞内探针用于器官环境监测的潜力.
主要方法:
- 选择性修改纳米棒 (NRs) 与DNA寡合体,以创建复杂的组件.
- 三种不同的异构体的制备:末端,侧面和卫星.
- 使用多种实验方法进行表征,以验证结构,均性和稳定性.
- 表面增强拉曼光谱 (SERS) 和电场模拟.
- 用HeLa细胞进行化,用于细胞内探针研究.
主要成果:
- 对于具有明显区域特异性的末端,侧面和卫星异构体,实现了高产量 (> 85%).
- 验证了组件的结构特征,统一性和稳定性.
- 证明SERS强度主要取决于上层结构中的NP数量.
- 展示了无标签NP-NR组件在活HeLa细胞中检测线粒体脂质和小代谢物的能力.
- 提供了对当地的有机体环境实时探测的概念验证.
结论:
- 开发了一种复杂的多功能合成路径,用于复杂的,DNA导向的等离子组件.
- 证明了这些组件作为敏感的细胞内探针用于器官分析的潜力.
- 突出了对多目标监控和先进光学应用的进一步结构优化的需求.
相关概念视频
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The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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