酵母介质程序的高分辨率图像,由核糖体造型分析揭示出来
Gloria A Brar1, Moran Yassour, Nir Friedman
1Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Science (New York, N.Y.)
|December 24, 2011
概括
这项研究表明,酵母半转化通过信使RNA (mRNA) 水平和蛋白质翻译效率密切控制基因表达. 这种调节会影响基因表达时间和细胞分裂期间的蛋白质产生.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 弥生是性繁殖的一个基本的生物过程,从双胞胎细胞产生单体性雌激体.
- 了解调节过程中的调节机制对于理解发育和遗传至关重要.
研究的目的:
- 研究酵母半转化过程中基因表达的动态,特别是信使RNA (mRNA) 的丰富性和蛋白质的产生.
- 识别涉及介质进展和细胞重塑的新型因素和细胞过程.
- 阐明翻译控制的作用,包括非正规的翻译事件,在介质基因表达中.
主要方法:
- 量化测量mRNA水平和蛋白质的生产,在整个酵母介质化程序.
- 随时监测蛋白质合成以确定表达时间.
- 对核糖体占用率和翻译启动位点的分析,包括上游开放阅读框架 (uORF).
主要成果:
- 大多数基因都表现出强烈的,特定阶段的表达模式,由mRNA水平和翻译效率控制.
- 在介质性进展过程中发现了非特征性的重组因子和显著的细胞器重塑.
- 梅奥特翻译经常发生在非正典网站上,例如在没有注释的转录和uORF上的短开放阅读框架 (ORF).
- 特定uORF的核糖体占用影响了下游ORF翻译的效率,其中一些AUG uORF具有对抗作用.
结论:
- 转化控制是酵母半转化过程中普遍和关键的调节机制.
- 这项研究揭示了细胞重组和基因表达动态中介细胞的分子基础的新见解.
- 这些发现突显了非正典翻译事件在编排复杂的发育过程,如半变化的重要性.
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