在脊椎动物的胚胎发育中保持了lincRNAs的功能,尽管有快速序列进化
Igor Ulitsky1, Alena Shkumatava, Calvin H Jan
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Cell
|December 27, 2011
概括
研究人员在斑马鱼中鉴定出超过550种长时间干预的非编码RNA (lincRNAs). 这些lincRNAs在胚胎发育中起着至关重要的作用,尽管物种间的序列相似性很小,但它们的功能仍然存在.
科学领域:
- 基因组学就是基因组学.
- 发展生物学 发展生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 在哺乳动物中已经确定了成千上万种长时间干预的非编码RNA (lincRNAs).
- 了解这些RNA的演化和功能至关重要.
研究的目的:
- 在斑马鱼中识别和描述lincRNAs.
- 在胚胎发育过程中调查斑马鱼lincRNAs的进化保护和功能作用.
主要方法:
- 使用染色体标记,多分子A位点映射和RNA-Seq数据用于lincRNA识别.
- 采用反感应试剂以准斑马鱼lincRNAs的保护区域和拼接部位.
- 评估了发育缺陷和使用成熟的lincRNA及其哺乳动物正义词的救援实验.
主要成果:
- 在斑马鱼中确定了超过550种不同的lincRNA,与哺乳动物lincRNA共享特征.
- 发现了与哺乳动物的正经相似性 (29/550) 的有限序列相似性,通常仅限于保存区域.
- 证明了准斑马鱼lincRNAs的保护区域或拼接部位会导致发育缺陷,这些缺陷被同类lincRNAs所挽救.
- 展示了一些lincRNAs没有序列保存的保存基因组位置.
结论:
- 斑马鱼的lincRNA在胚胎发育中起着至关重要的作用.
- lincRNA的功能在物种之间得到保留,尽管序列的保护有限.
- 这项研究为模型生物中的lincRNA识别和分析提供了一个框架.
相关概念视频
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