通过全基因组定位分析在人类细胞中发现的染色质调节者的组合模式
Oren Ram1, Alon Goren, Ido Amit
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Cell
|December 27, 2011
概括
我们开发了ChIP-string来绘制全基因组的染色质调节器 (CRs). CRs以特定的组合与不同的基因组区域结合,揭示了它们在不同细胞类型中的功能原理.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 染色体调节剂 (CRs) 对于通过基因素修饰来控制基因表达至关重要.
- 控制CR功能的精确规则及其组合相互作用在很大程度上是未知的.
- 了解CRs对于破译基因调节和细胞过程至关重要.
研究的目的:
- 开发一种系统的方法来推断数百种染色体调节者的功能.
- 创建一种用于绘制CRs全基因组结合的新型试验.
- 揭示结合式CR功能的原理及其在不同染色体环境中的组织.
主要方法:
- 开发了ChIP-string,这是一种将染色质免疫沉与签名读取结合在一起的中等尺度测试.
- 选了145种抗体,以确定用于CR映射的有效试剂.
- 在两个不同的细胞类型中绘制了29个CRs的全基因组结合图.
主要成果:
- 在特定的染色体环境中确定了CR组合的特征性同位素化模式.
- 观察到CRs与连贯功能的基因和远端调节元件有关.
- 证明CRs在保持模块化和组合性关联的同时,在细胞类型之间重新分布到不同的位置.
结论:
- ChIP-string提供了一种多重复合方法,以加强对CR结合的监测.
- 这项研究提供了大量的CR全基因组结合图的资源.
- 揭示了控制染色体调节者的组合功能的共同原则.
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