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Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
在RNA结合和特异性中单链结的作用,由甲基 I 组的 ribozyme
Xuesong Shi1, Sergey V Solomatin, Daniel Herschlag
1Department of Biochemistry, Stanford University, Stanford, California 94305, USA.
Journal of the American Chemical Society
|January 7, 2012
概括
铁胺组I ribozyme 中的J1/2 RNA 结对催化活性产生显著影响. 一种特定的腺 (A29) 稳定了基质结合P1双重体,揭示了相关内核中保存的分子统治机制.
科学领域:
- 分子生物学分子生物学
- RNA 生物化学 生物化学
- 结构生物学 结构生物学
背景情况:
- I 组的内子是自剪接RNAs.
- 甲基核糖酶是研究RNA催化的一个模型系统.
- RNA结点在RNA结构和功能中起着关键的作用.
研究的目的:
- 为了研究J1/2RNA结的功能作用,在Tetrahymena组I ribozyme.
- 为了确定J1/2如何影响催化活性,基质P1双重动力学和对接热力学.
- 阐明 J1/2 在 ribozyme 函数中的序列依赖性.
主要方法:
- 单旋转动力学测试. 单旋转动力学测试.
- 光异质性测量. 光异质性测量.
- 单分子弗斯特尔共振能量转移 (smFRET) 研究.
- 在J1/2区域的位点定向突变发生.
主要成果:
- 在J1/2中的突变,特别是在腺A29中的突变,显著改变了 ribozyme 活性 (高达 2 个数量级).
- 通过与螺旋P2 (A31·U56) 的三级相互作用,A29稳定了P1重复的对接状态.
- 序列分析揭示了一种保存的"分子统治者"机制,涉及A29在I组内部子类中的5'-拼接位点选择.
结论:
- J1/2连接对四胺组I рибо酶功能至关重要,从一个简单的RNA连接中显示出显著的功能效应.
- 像A29这样的结核酸介导的三级相互作用对于稳定催化构造至关重要.
- RNA结序列可以快速演变,以调解RNA功能所必需的特定相互作用,例如基质定位.
相关概念视频
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acids
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
