在Pr光异构化过程中实时跟踪植物染色体的方向变化
Yang Yang1, Martin Linke, Theodore von Haimberger
1Department of Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany.
Journal of the American Chemical Society
|January 11, 2012
概括
光诱导的植物染色体的Z-to-E光异构化涉及D环在激发状态中的30秒旋转. 这一对于光受体信号传递至关重要的过程是由单个旋转和蛋白质相互作用驱动的.
科学领域:
- 生物物理学的生物物理.
- 摄影化学的使用.
- 分子生物学分子生物学
背景情况:
- 与蛋白质结合的染色体光异构化是光受体功能的基础.
- 植物染色体是各种生物体中关键的感光蛋白.
研究的目的:
- 为了研究 Pr Cph1Δ2 植物染色体的 Z-to-E 光异构化机制.
- 为了确定光异构化过程的结构动态和时间常数.
主要方法:
- 使用极化分辨率的秒可见红外探针光谱学.
- 分析了光反应动力学和激发和基态的结构变化.
主要成果:
- 确定了两个光反应时间常量:4.7 ± 1.4 ps和30 ± 5 ps.
- 在激发状态下观察到D环的实时旋转,时间常数为30±5秒.
- 完成的环D旋转发生在电子激发状态下.
结论:
- 光异构化是通过在Pr*状态中围绕C(15) C(16) 甲桥的一次旋转来启动的.
- 这个过程涉及与周围蛋白质环境的扩散相互作用.
- 这些发现阐明了控制植物色信号的超快速结构动态.
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