通过突变剖析识别的DOC2蛋白质对于虫复合体寄生虫外细胞形成至关重要
Andrew Farrell1, Sivasakthivel Thirugnanam, Alexander Lorestani
1Department of Biology, Boston College, Chestnut Hill, MA 02467, USA.
Science (New York, N.Y.)
|January 17, 2012
概括
DOC2 蛋白质对于复合体寄生虫外细胞形成至关重要. 在Toxoplasma gondii TgDOC2.1中发生的突变破坏了微膜分泌,影响了宿主细胞的入侵和退出.
科学领域:
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 外细胞分裂对于包括Toxoplasma gondii和Plasmodium falciparum在内的杂寄生虫的性循环至关重要.
- DOC2蛋白调节膜融合在异细胞形成过程中,以一种依赖的方式.
研究的目的:
- 为了研究TgDOC2.1在Toxoplasma gondii病变发生中的作用.
- 为了确定DOC2蛋白在复合体寄生虫外细胞和入侵中的功能.
主要方法:
- 产生了一种有条件突变的Toxoplasma gondii,对宿主细胞的入侵和退出有损.
- 进行全基因组测序以确定TgDOC2.1.1中的突变.
- 设计了Plasmodium falciparum中正基因的条件等位基因.
主要成果:
- 突变的Toxoplasma gondii表现出微膜分泌中的缺陷.
- 全基因组测序确定了TgDOC2.1.的致病突变.
- 杆菌的正义检测也显示出缺陷的微膜分泌物,主要影响了入侵.
结论:
- 在Toxoplasma gondii中,TgDOC2.1对于微膜分泌是必不可少的,影响了侵入和退出.
- 微膜分泌对于Toxoplasma gondii的发病过程至关重要.
- 微膜分泌似乎是Plasmodium falciparum出口的必需品,在入侵中起着主要作用.
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