含有聚乳酸的纳米颗粒的可降解性:通过交联的外屏障进行酶性访问
Sandani Samarajeewa1, Ritu Shrestha, Yali Li
1Department of Chemistry, Texas A&M University, College Station, Texas 77842, USA.
Journal of the American Chemical Society
|January 20, 2012
概括
纳米颗粒聚乳酸 (PLA) 芯的酶降解速度比散装PLA要快得多. 即使有交叉链接,这些可降解的纳米结构也显示出药物输送和生物清除的潜力.
科学领域:
- 聚合物科学 聚合物科学
- 纳米技术 纳米技术
- 生物材料是一种生物材料.
背景情况:
- 聚乳酸 (PLA) 是一种水解可降解的聚合物.
- 核心块共聚合物微粒为纳米医学提供可调节的特性.
- 了解纳米粒子降解对于生物医学应用至关重要.
研究的目的:
- 为了比较散装PLA的酶化水解速率与区块共聚合物微粒内的PLA核.
- 为了调查PLA核心在米塞尔中的可访问性,即使有外交叉连接.
- 探索纳米尺寸对聚合物可降解性和蛋白质相互作用的影响.
主要方法:
- 使用蛋白酶K的酶性水解.
- 核磁共振 ((1) H NMR) 光谱仪用于动力分析.
- 使用NMR,光谱学,动态光散射 (DLS),原子力显微镜 (AFM) 和传输电子显微镜 (TEM) 的表征.
主要成果:
- 与散装PLA相比,纳米颗粒PLA芯表现出明显加快的酶性水解.
- 大约70%的PLA核心在48小时内降解为非交叉链接的菌体.
- 的交叉连接稍微减少了核心降解到大约50%,这表明可访问性得到保留.
结论:
- 纳米尺寸显著提高了PLA的酶降解性.
- 区块共聚合物微粒内的PLA核心仍然可以降解,即使外是交叉连接的.
- 这些发现支持可降解纳米结构的开发,以控制治疗释放和生物清除.
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