广泛的促进者中心的染色体相互作用为转录调节提供了拓基础
Guoliang Li1, Xiaoan Ruan, Raymond K Auerbach
1Genome Institute of Singapore, Singapore 138672, Republic of Singapore.
Cell
|January 24, 2012
概括
这项研究揭示了使用染色体相互作用分析与配对终标签测序 (ChIA-PET) 绘制的更高阶染色体相互作用如何调节人类细胞中的基因转录,影响一般和特定的基因活性.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 基因组学就是基因组学.
背景情况:
- 了解高阶染色体组织对于破译真核细胞转录调节至关重要.
- DNA的空间布局影响基因表达,但仍然不完全理解.
研究的目的:
- 在人类细胞中绘制与RNA聚合酶II相关的长距离色素相互作用.
- 研究这些相互作用在对家庭和细胞特异性基因的转录控制中的作用.
- 探索染色体相互作用和与疾病相关的遗传元素之间的关系.
主要方法:
- 使用全基因组染色体相互作用分析与配对末端标签测序 (ChIA-PET).
- 分析的重点是人类细胞中涉及RNA聚合酶II的相互作用.
- 进行了不同细胞系的比较分析.
主要成果:
- 广泛发起者中心的内基因,外基因和基因间相互作用被确定.
- 相互作用聚合到更高阶的集群中,形成近端和远端基因之间的促销者-促销者相互作用.
- 大多数具有促销者-促销者相互作用的基因都被积极转录,这表明合作调节和组合转录控制的潜力.
- 观察到细胞特异性染色质相互作用,为细胞特异性转录和增强剂-促进剂参与提供框架.
- 发现与疾病相关的非编码元素与相应的基因空间相互作用.
结论:
- 三维色素相互作用在调节人类的家政和细胞特异性基因方面发挥着重要作用.
- 这项研究为通过更高阶染色体组织对转录控制的结构基础提供了新的见解.
- 染色体相互作用将非编码遗传元素与基因调节联系起来,并可能对理解遗传疾病产生影响.
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