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相关概念视频

Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...

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相关实验视频

Updated: May 25, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

可编程的一多步骤有机合成,使用DNA结.

Mireya L McKee1, Phillip J Milnes, Jonathan Bath

  • 1Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU, UK.

Journal of the American Chemical Society
|January 27, 2012
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的DNA模板合成系统,用于控制的寡合物生产. 该方法可以在单个中实现高效的并行合成,减少复杂分子的反应步骤.

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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

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相关实验视频

Last Updated: May 25, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 合成化学 合成化学

背景情况:

  • 寡合物的多步合成是复杂的,通常需要多个反应步骤和净化阶段.
  • 目前的方法可能受到反应条件随着寡合体长度的变化而受到限制.
  • 并行合成和结合策略可以减少整体合成时间,但需要仔细控制.

研究的目的:

  • 开发一种用于多步DNA模板合成的新系统.
  • 为了使序列控制的寡合物合成在一个恒定的反应环境中.
  • 为了在单个反应容器内促进并行合成和结合.

主要方法:

  • 利用DNA连接的顺序形成来控制合成过程.
  • 将反应物附着在DNA适配器上,这些适配器将其混合到DNA模板链上.
  • 在代合成中重复杂化和反应步骤.
  • 在一个单一的中进行合成,其中包含所有必要的反应性单体.
  • 来自并行合成的结合产品,以减少整体步骤.

主要成果:

  • 展示了一种可控的多步骤DNA模板合成系统.
  • 在恒定的反应环境下实现了序列控制的寡合物合成.
  • 能够在同一容器中并行合成不同的寡合物.
  • 展示了并行合成产品的结合,以减少步骤.

结论:

  • 开发的DNA模板系统为高效的寡合物合成提供了一个强大的平台.
  • 该方法允许在单个中进行序列控制和并行处理.
  • 这种方法简化了复杂的合成,减少了反应步骤,提高了生产力.