顺序信号交叉调节了海胚胎中的内体皮分离
Aditya J Sethi1, Radhika M Wikramanayake, Robert C Angerer
1National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
Science (New York, N.Y.)
|February 4, 2012
概括
痕信号和Wnt信号一起工作,将胚胎内皮分为内皮和中皮. 这个过程涉及Notch在中皮层中抑制内皮层基因和Wnt加强内皮层命运.
科学领域:
- 发育生物学 发展生物学
- 细胞信号传递 细胞信号传递
- 遗传学 是一个遗传学.
背景情况:
- 控制胚胎内皮体内皮体分离成不同的内皮体和中皮体系的精确机制在deuterostomes仍然不完全理解.
- 了解这种基本的发育过程对于理解早期胚胎发生和潜在的发育障碍至关重要.
研究的目的:
- 阐明分子通路和信号相互作用,调节内分体胚胎内分体分离.
- 调查Notch和Wnt信号在建立单独的内皮和中皮命运中的作用.
主要方法:
- 利用海胚胎作为研究早期胚胎发育的模型系统.
- 采用分子生物学技术来分析基因表达模式和蛋白质定位.
- 研究了Endomesoderm分化过程中Notch信号和Wnt信号通路之间的相互作用.
主要成果:
- 证明了诺奇信号通过抑制假定中皮的关键内皮转录因子来启动内皮分离.
- 确定了一个调节电路,其中内皮转录因子维持了正规的Wnt (cWnt) 配体表达,特别是在内皮前体中.
- 表明在中皮层中β-catenin转录协激剂的Notch-依赖核出口使其对cWnt信号无反应,从而使其与内皮的命运隔绝.
结论:
- 细胞内膜分离是一个渐进的,多步骤的过程,由Notch和Wnt信号通路之间的顺序相互作用协调.
- 这项研究揭示了一种新的调节机制,可以控制细胞在早期deuterostome发育过程中的命运决定.
- 这些发现为细菌层规范的分子基础提供了关键的见解.
相关概念视频
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
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02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:51Determination
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01:56Gastrulation
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...