一个转录因子集体定义了心脏细胞的命运,并反映了血统历史
Guillaume Junion1, Mikhail Spivakov, Charles Girardot
1Genome Biology Unit, European Molecular Biology Laboratory, D-69117 Heidelberg, Germany.
Cell
|February 7, 2012
概括
在Drosophila中,心脏特异性涉及转录因子 (TF) 和信号通路. 这些TF协同调节心脏增强剂的活性,显示动机灵活性和潜在的分子足迹,用于发育谱系.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 细胞命运决定整合了诱导信号和转录因子 (TF).
- 对于细胞特异性基因表达的cis-regulatory元素的TF收机制尚未完全理解.
- 了解心脏特异性需要阐明遗传组件如何协调增强剂活性.
研究的目的:
- 调查多索菲拉在心脏特异性中必不可少的遗传成分如何合作调节心脏增强剂活性.
- 探索结合式TF约束的约束灵活性和功能影响.
- 在cis调节元素中识别发育谱系的潜在分子特征.
主要方法:
- 在体内和体外测试以评估心脏增强剂活性.
- 对五个关键遗传组件用于心脏特异性的组合结合的分析.
- 位点定向突变发生,以调查特定TF结合位点的作用.
主要成果:
- 心脏特征的五个遗传成分作为一个合作单位来调节心脏增强剂活动.
- 结合式TF绑定显示出广泛的图案灵活性,独立于方向或间距.
- 一组增强剂在内脏半皮表现出活性,可以通过单位突变重定向到心脏.
结论:
- 配合TF结合与动图灵活性是调节心脏增强剂活性的一个关键机制.
- 相关组织中的"休眠"TF结合签名可能反映了共同的发育起源.
- 这项研究提供了关于细胞命运决定和发育谱系的调控逻辑的见解.
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