通过MicroRNA-JAK/STAT电路调节昼夜行为输出
1Cell and Molecular Biology Program, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Cell
|February 7, 2012
概括
微RNAmiR-279通过调节JAK/STAT通路来控制果的日常休息:活动节奏,作用于中央昼夜时钟的下游,影响行为.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 生物钟的分子机制已知,但它们如何产生节律行为仍然不清楚.
- 了解来自昼夜时钟的信号传输对于解释行为节奏至关重要.
研究的目的:
- 研究微RNAmiR-279在调节Drosophila的昼夜节律和行为中的作用.
- 阐明通过miR-279影响休息:活动模式的分子途径.
主要方法:
- 在Drosophila中对miR-279表达 (过度表达和删除) 的基因操纵.
- 休息的分析:活动节奏和昼夜行为.
- 研究JAK/STAT信号通路,包括连接体Upd.
- 检查时钟神经元和UPD表达神经元之间的关系.
主要成果:
- miR-279调节JAK/STAT信号传递以驱动Drosophila的休息:活动节奏.
- 改变miR-279水平 (过度表达或删除) 会破坏这些节奏.
- miR-279 作用于中央昼夜时钟的下游,因为 PERIOD 蛋白质的振荡保持正常.
- 该JAK/STAT连接体Upd是miR-279的直接目标;它的敲击拯救了miR-279突变表型.
- 调节JAK/STAT通路影响昼夜节律,中央时钟神经元可能与UPD表达神经元进行物理相互作用.
结论:
- 微RNA miR-279 是Drosophila中昼夜休息:活动节律的关键调节者.
- 该miR-279/JAK/STAT通路为中央昼夜时钟提供了一种机制来控制行为.
- 这项研究揭示了微RNA,JAK/STAT通路和昼夜节律生成之间的新联系.
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