铜类固醇通过GSK3介导的MAPK通路的抑制来调节口腔发育
Tae-Wuk Kim1, Marta Michniewicz, Dominique C Bergmann
1Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305-4150, USA.
Nature
|February 7, 2012
概括
布拉西诺固醇通过调节一个关键的信号通路来抑制植物口腔发育. 这项研究揭示了布拉辛固醇如何缓解基因激活蛋白激酶 (MAPK) 模块的抑制,影响光合作用和用水.
科学领域:
- 植物生物学 植物生物学
- 分子信号传递是分子信号传递.
- 发育生物学是发展生物学.
背景情况:
- 胃体的发育对于植物的光合作用和用水效率至关重要.
- 线素激活蛋白激酶 (MAPK) 信号通路在调节口腔形成中起着至关重要的作用.
- 控制口腔发育的精确信号网络仍然不完全理解.
研究的目的:
- 为了研究布拉西诺固醇在调节阿拉比多普西斯 (Arabidopsis) 的口腔发育中的作用.
- 阐明布拉西诺固醇在口腔发育中影响MAPK信号的分子机制.
- 为了确定brassinosteroid介导的口腔发育的上游调节者和下游目标.
主要方法:
- 在Arabidopsis.中进行遗传分析.
- 在体外和体内生物化学测定.
- 酸化试验用于研究蛋白质相互作用.
主要成果:
- 铜类信号通过类似于糖原合成酶激酶3 (GSK3) 的激酶BIN2抑制口腔发育,这种激酶在MAPK激酶激酶 (MAPKKK) YDA.上游起作用.
- BIN2可酸化YDA,抑制其对MKK4的酸化.
- 黄类固醇治疗或GSK3抑制会增加MAPK活性,而黄类固醇缺乏会减少MAPK活性.
结论:
- 铜类固醇通过缓解YDA-MKK4 MAPK模块的GSK3介导抑制来抑制口腔发育.
- 这项研究通过特定的MAPK途径建立了布拉西诺类信号与口腔发育之间的联系.
- 这些发现提供了关于植物生物化学和解剖学的协调的见解,以优化光合作用和利用水的效率.
相关概念视频
02:04Regulation of Transpiration by Stomata
26.3K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
26.3K
01:57Adaptations that Reduce Water Loss
24.4K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
24.4K
01:25Cell Signaling in Plants
4.5K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
4.5K
01:07MAPK Signaling Cascades
7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
01:20The JAK-STAT Signaling Pathway
10.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
10.2K
01:17Gene Regulation During Sporulation
719
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
719