相关实验视频
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High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
一个下游启动元件,对于高效的TATA盒绑定和TFIID的体外功能而言是必不可少的
Y Nakatani1, M Horikoshi, M Brenner
1Laboratory of Molecular Biology, NINDS, National Institutes of Health, Bethesda, Maryland 20892.
Nature
|November 1, 1990
概括
质纤维酸蛋白 (gfa) 基因促进器需要TATA盒和下游启动元件才能有效转录. 自然的人类TFIID与克隆版本不同,结合了这两种元素,这表明额外的因素增强了转录启动.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 神经科学是一个神经科学.
背景情况:
- 质纤维酸蛋白 (gfa) 基因编码了质细胞中的一个关键的中间丝蛋白.
- 促使者分析揭示了人类gfa基因中的两个不同的启动元素:一个TATA盒子和一个下游启动元件.
研究的目的:
- 调查gfa发起者的TATA盒子和下游启动元件在转录中的功能作用.
- 描述自然人类TFIID和克隆TFIID对这些促进元素的结合性.
主要方法:
- 转染试验被用来评估在培养细胞中转录的促进子元素的要求.
- 实验室转录试验和DNase足迹测试使用部分纯化的天然人类TFIID和细菌表达的克隆TFIID进行.
- 进行了gfa促进物的删除分析.
主要成果:
- 塔塔盒和下游启动元件对于高效的gfa基因转录至关重要.
- 自然的人类TFIID结合TATA盒和下游启动器,而克隆的人类和酵母TFIID主要结合TATA盒.
- 下游启动器的删除损害了TFIID与TATA盒的结合,并减少了转录.
- 克隆的TFIID对TATA盒绑定和转录的下游启动器的依赖性较小.
结论:
- 为了高效地转录gfa基因,需要TATA盒和下游发起者的合作行动.
- 自然的人类TFIID可能具有与下游发起者相互作用的额外组件,稳定TFIID结合并促进转录.
相关概念视频
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The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

