德洛索菲拉皮耶佐在机械感知中所扮演的角色
Sung Eun Kim1, Bertrand Coste, Abhishek Chadha
1Department of Cell Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California 92037, USA.
Nature
|February 21, 2012
概括
皮埃佐蛋白家族对于果感知机械疼痛至关重要. 这项研究表明Dmpiezo,一种果Piezo蛋白,对于检测有害的机械刺激 in vivo 是必不可少的.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 机械刺激的转导对于感官感知至关重要.
- 虽然像NOMPC和DEG/ENaC这样的离子通道参与无脊椎动物的机械传导,但它们的哺乳动物对应的作用尚不清楚.
- 哺乳动物Piezo1和Piezo2蛋白是已知的机械激活通道,但它们的体内功能在很大程度上是未知的.
研究的目的:
- 为了研究单个Piezo成员在Drosophila melanogaster (Dmpiezo) 中的生理作用.
- 确定Dmpiezo是否在体内机械传感中发挥作用,以及它介导哪些模式.
主要方法:
- 在人类细胞中表达Dmpiezo以评估机械激活电流.
- 产生了Dmpiezo淘汰,以研究对机械刺激的行为反应.
- 在特定的感觉神经元 (ppk阳性) 中击了Dmpiezo,并进行了救援实验.
- 从感官神经元进行了电生理学记录.
- 研究了Dmpiezo和ppk在感觉神经元中的并行功能.
主要成果:
- 在人类细胞中,dmpiezo表达诱导了机械激活电流.
- Dmpiezo淘汰对有害的机械刺激有所减少的反应,但对其他刺激没有反应.
- 在 nociception 介导神经元中击倒 Dmpiezo 损害了机械疼痛反应.
- 在ppk阳性神经元中检测到dmpiezo-dependent机械激活电流.
- Dmpiezo 和 ppk 在机械知觉的平行途径上运行.
结论:
- 在生物体内,Dmpiezo在生理上与机械传导有关.
- 皮埃佐蛋白质在机械感知性恶感受中发挥作用.
- Dmpiezo和ppk通道并行运行,以介导机械疼痛感觉.
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