Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Efficient <sup>15</sup>N hyperpolarization of [<sup>15</sup>N<sub>3</sub>]metronidazole antibiotic via spin-relayed pulsed SABRE-SHEATH.

Journal of magnetic resonance open·2026
Same author

Correction to "Low-Cost Purpose-Built Ultra-Low-Field NMR Spectrometer".

Analytical chemistry·2026
Same author

Correction to "Carbon-13 Radiofrequency Amplification by Stimulated Emission of Radiation of the Hyperpolarized Ketone and Hemiketal Forms of Allyl [1-<sup>13</sup>C]Pyruvate".

ACS sensors·2026
Same author

Correction to "<sup>15</sup>N NMR Signal Amplification by Reversible Exchange of [<sup>15</sup>N<sub>3</sub>]Ornidazole Antibiotic".

The journal of physical chemistry. B·2026
Same author

Correction to "Perfluorinated Iridium Catalyst for Signal Amplification by Reversible Exchange Provides Metal-Free Aqueous Hyperpolarized [1-<sup>13</sup>C]-Pyruvate".

Journal of the American Chemical Society·2026
Same author

Correction: SABRE-SHEATH hyperpolarized <sup>15</sup>N<sub>2</sub>-imidazole for Zn<sup>2+</sup> sensing.

Chemical communications (Cambridge, England)·2026

相关实验视频

Updated: May 24, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

帕萨德纳超极化13C酸盐酸盐

Roman V Shchepin1, Aaron M Coffey, Kevin W Waddell

  • 1Department of Radiology, Vanderbilt University Institute of Imaging Science (VUIIS), Nashville, Tennessee 37232, USA.

Journal of the American Chemical Society
|February 23, 2012
PubMed
概括
此摘要是机器生成的。

1-(13) C-烯酸被超极化为1-(13) C-酸盐,使用和合成可显著增强核对齐 (PASADENA). 这使得在3T时对13C极化产生了4000倍的灵敏度增强.

更多相关视频

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Published on: July 8, 2025

相关实验视频

Last Updated: May 24, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Published on: July 8, 2025

科学领域:

  • 核磁共振 (NMR) 光谱学 核磁共振 (NMR) 光谱学
  • 超极化技术 超极化技术
  • 化学物理 化学物理

背景情况:

  • 核旋转超极化显著提高了NMR信号的灵敏度.
  • 超诱导极化 (PHIP) 是超极化的一个关键方法.
  • 开发高效的超极化方法对于推进NMR应用至关重要.

研究的目的:

  • 为了证明1-(13) C-酸盐从1-(13) C-酸盐的高极化.
  • 报告超极化产品的 (13) C 旋转放松时间 (T1).
  • 要量化使用PASADENA技术实现的灵敏度增强.

主要方法:

  • 使用了PASADENA (超和合成允许大幅增强的核对齐) 技术进行超极化.
  • 采用了完全质子化的前体的分子化.
  • 在3特斯拉进行NMR光谱测量,以测量极化水平和放松时间.

主要成果:

  • 成功转化1-(13) C-基酸为高极化1-(13) C-酸盐.
  • 对超极化产品实现了36秒的 (13) C T1 放松时间.
  • 证明了1%的 (13) C极化水平,在3T时产生了约4000倍的灵敏度增强.

结论:

  • 帕萨迪纳技术使特定分子的显著超极化成为可能.
  • 这种方法为13C观测提供了NMR灵敏度的显著增加.
  • 报告的研究结果代表了使用这种方法进行增强型NMR光谱学的原则证明.