在Bacillus subtilis代谢的动态适应过程中,全球网络重组
Joerg Martin Buescher1, Wolfram Liebermeister, Matthieu Jules
1Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
Science (New York, N.Y.)
|March 3, 2012
概括
细菌细菌对酸盐和葡萄糖的适应不同,酸盐的适应是快速的和转录后的,而葡萄糖的适应是缓慢的和转录的. 复杂的监管网络受到进化权衡的青.
科学领域:
- 微生物生理学和系统生物学.
- 细菌适应的分子机制.
- 代谢和调节网络的相互作用.
背景情况:
- 细胞适应环境变化涉及复杂的代谢和调节网络相互作用.
- 以前的研究往往集中在有限的监管层,阻碍了全面的理解.
- 了解Bacillus subtilis适应不同碳来源对微生物生物技术至关重要.
研究的目的:
- 研究和比较Bacillus subtilis在适应不同碳来源 (酸盐与葡萄糖) 期间的多层调节策略.
- 阐明在营养转换期间的转录,后转录和代谢调节之间的相互作用.
- 为了确定有利于复杂的监管控制计划的进化驱动因素.
主要方法:
- 动态转录组学,蛋白组学,代谢组学和促进体活动数据的综合分析.
- 应用基于统计和模型的方法来解释数据.
- 对Bacillus subtilis适应马拉酸盐和葡萄糖的比较分析.
主要成果:
- 适应酸盐是快速的,并且主要通过转录后控制.
- 适应葡萄糖的速度较慢,主要在转录层面进行调节,涉及监管网络的很大一部分.
- 跨多个监管层次的相互作用有助于适应性变化,这也可以通过单基因控制来实现.
结论:
- 细菌细菌采用不同的调节策略来适应不同的碳来源,突出突出细胞反应的环境依赖性.
- 转录后调节促进了快速适应,而转录控制则是缓慢,更广泛的代谢转变的关键.
- 进化中的权衡和约束可能促使选择复杂的监管程序,以实现强大的细胞适应.
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