花的ABC蛋白控制着依赖于strigolactone的共生信号传递和分支
Tobias Kretzschmar1, Wouter Kohlen, Joelle Sasse
1Institute of Plant Biology, University of Zurich, 8008 Zurich, Switzerland. t.kretzschmar@irri.org
Nature
|March 9, 2012
概括
斯特里戈拉克顿调节植物的发育和共生. 这项研究确定了ABC输送器PDR1作为第一个已知的排球活素输出者,对于根到芽信号和共生相互作用至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子植物生理学分子植物生理学
- 农业科学 农业科学
背景情况:
- 斯特里戈拉克顿是调节共生相互作用的植物激素和射击架构.
- 它们的运输机制在很大程度上是未知的,这阻碍了对它们的功能的理解.
- ATP结合盒 (ABC) 载体参与激素转位.
研究的目的:
- 为了确定负责strigolactone运输的分子组件.
- 为了阐明ABC载体在strigolactone转移中的作用.
- 为了调查Petunia杂交PDR1在strigolactone出口中的功能.
主要方法:
- 培杂交的特征 pdr1突变.
- 分析从根源中排出的格拉克.
- 射击架构的表型分析 (分支).
- 在Arabidopsis thaliana中对Petunia axillaris PDR1的功能分析.
主要成果:
- 佩图尼亚pdr1突变体在strigolactone排泄中表现出缺陷,并减少了状菌根共生.
- pdr1突变体表现出增强的小支部分支,这表明strigolactone分配受损.
- 在Arabidopsis中,PDR1的过度表达赋予了对合成杆菌素的耐受性,这表明出口的增强.
结论:
- 花杂交PDR1功能作为一个细胞杆菌素的出口者.
- PDR1在调节状菌根和射击架构方面发挥着关键作用.
- 这一发现提供了第一个已知的strigolactone运输的组成部分,为操纵打开了大道.
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