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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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相关实验视频

Updated: May 24, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

用激光诱导的电子衍射成像超快的分子动力学.

Cosmin I Blaga1, Junliang Xu, Anthony D DiChiara

  • 1Department of Physics, The Ohio State University, Columbus, Ohio 43210, USA. cblaga@mps.ohio-state.edu

Nature
|March 9, 2012
PubMed
概括

激光诱导电子衍射 (LIED) 能够实现超快速的分子成像. 这种新方法以5秒的精度捕捉分子中的原子结构变化,推进分子动力学研究.

科学领域:

  • 物理,化学和生物学
  • 超快速科学 超快速科学
  • 分子成像学分子成像学

背景情况:

  • X射线和电子衍射是确定分子结构的关键.
  • 目前的衍射方法仅限于皮秒时间尺度.
  • 五秒钟源提供了超快速分子快照的潜力.

研究的目的:

  • 为了证明激光诱导的电子衍射 (LIED) 用于分子成像.
  • 为了实现低于波的精度和五秒钟的曝光时间.
  • 为了图像分子结构动力学.

主要方法:

  • 利用连贯电子波包的激光电离爆发进行自我审讯.
  • 使用中红外波长 (1.7,2.0,2.3μm) 对氧和分子应用LIED.
  • 从光电子运动量分布中提取衍射图案.

主要成果:

  • 实现了sub-ångström空间分辨率和几秒钟的曝光时间.
  • 经证明的灵敏度可以测量0.1 Å的氧键长度位移在~5 fs内.
  • 展示了波长变化作为在不同时间捕获快照的手段.

结论:

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  • LIED 是一种有前途的技术,用于成像气相分子.
  • 为分子动力学提供前所未有的时空分辨率.
  • 能够详细研究分子中超快的结构变化.