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Updated: May 24, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

用RNA对细胞代谢物进行光成像

Jeremy S Paige1, Thinh Nguyen-Duc, Wenjiao Song

  • 1Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10065, USA.

Science (New York, N.Y.)
|March 10, 2012
PubMed
概括

研究人员使用菜RNA开发了新的光传感器,以图像化细胞内的小分子. 这种基于RNA的多功能方法为检测各种细胞生物分子提供了一种新方法.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...