DBIRD复合体集成了替代mRNA剪接与RNA聚合酶II转录延长的替代mRNA剪接
Pierre Close1, Philip East, A Barbara Dirac-Svejstrup
1Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms EN6 3LD, UK.
Nature
|March 27, 2012
概括
这种新型的DBIRD复合物将RNA聚合酶II (RNAPII) 与信使核糖蛋白 (mRNP) 粒子结合起来. 这个复合体将转录延长与替代拼接集成在一起,特别是在 (A+T) 丰富的DNA中的外子.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 替代信使RNA (mRNA) 拼接从有限的基因组中产生蛋白质组复杂性.
- 拼接与转录相结合,并受到转录延长率的影响.
- 转录RNA聚合酶II (RNAPII) 和信使核糖蛋白 (mRNP) 颗粒之间的联系尚不清楚.
研究的目的:
- 为了描述染色质相关的mRNP粒子的人类互动体.
- 为了确定关联RNAPII,mRNP粒子和拼接调节的因素.
- 阐明这些因素在整合转录和拼接中的作用.
主要方法:
- 对与染色质相关的mRNP粒子进行人间互动组分析.
- 识别和描述与RNAPII相互作用的蛋白质复合体.
- 通过RNA干扰 (RNAi) 介导的枯竭来评估功能影响.
- 对转录延长率和替代拼接模式的分析.
主要成果:
- 确定了含有DBC1和ZNF326 (ZIRD) 的DBIRD复合体,作为一种新的RNAPII交互因子.
- DBIRD直接与RNAPII结合,并局部化到受影响的外因子.
- DBIRD调节了 (A+T) 丰富的DNA中的异构体的替代拼接.
- DBIRD 枯竭导致转录延长的区域特异性下降.
结论:
- DBIRD复合体在mRNP粒子和RNAPII的接口上起作用.
- DBIRD将转录延长与替代拼接的调节相结合.
- 这种机制有助于哺乳动物的蛋白质组复杂性.
相关概念视频
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...


