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合作的三级交互网络指导RNA折叠.
Reza Behrouzi1, Joon Ho Roh, Duncan Kilburn
1T.C. Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
Cell
|April 17, 2012
概括
非编码RNA通过合作相互作用实现独特的3D结构,而不仅仅是个别的三级接触. 这种合作性引导RNA折叠,抑制不正确的结构,形成稳定,原生状态.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 非编码RNA折叠成复杂的3D结构,对它们的调节功能至关重要.
- 了解RNA的折叠机制,特别是三级相互作用的作用,至关重要.
研究的目的:
- 研究非编码RNA如何实现具有有限的三级相互作用动机的独特3D结构.
- 阐明合作性在RNA折叠和原生状态的形成中的作用.
主要方法:
- 在一组I ribozyme 中的第三级相互作用的位点导向突变发生.
- 微角X射线散射 (SAXS) 来探测结构性扰动.
- 酶活性测定,基足迹和原生聚烯胺凝电泳 (PAGE) 来评估折叠性和稳定性.
主要成果:
- 大多数三级相互作用对原生状态的稳定性影响最小.
- 在可折叠的中间产品中观察到核心和外围结构动机之间的合作联系.
- 这种合作性取决于本地螺旋方向,并抑制了非本地结构.
结论:
- RNA折叠是由一个新兴的合作互动网络指导的,而不仅仅是个别的三级互动.
- 合作性很可能是为了确保在非编码RNA中形成独特而稳定的本地折叠而演变的.
相关概念视频
RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
DNA has a double-helix structure. The...
Protein Folding
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Protein Folding
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