相关实验视频
Updated: May 23, 2026

09:52
Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
Published on: March 12, 2014
一个PLETHORA-auxin转录模块通过MAP65和CLASP控制细胞分裂平面的旋转
Pankaj Dhonukshe1, Daan A Weits, Alfredo Cruz-Ramirez
1Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. p.b.dhonukshe@uu.nl
Cell
|April 17, 2012
概括
植物干细胞分裂方向是由PLETHORA转录因子控制的,这些转录因子激活了auxin信号传递. 这对MAP65蛋白进行上调,重定向微管,用于精确的细胞分裂平面开关.
科学领域:
- 植物生物学 植物生物学
- 细胞力学 细胞力学
- 发育生物学是发展生物学.
背景情况:
- 定向细胞分裂对植物发育至关重要,但其调节的理解不够深.
- 植物的干细胞调节依赖于对细胞分裂平面的精确控制.
研究的目的:
- 阐明控制Arabidopsis干细胞中定向细胞分裂的分子机制.
- 为了确定上游因素和下游细胞组件参与划分平面重定位.
主要方法:
- 研究了PLETHORA转录因子和auxin信号传递的作用.
- 分析了微管相关的MAP65蛋白的表达和功能.
- 检查了CLASP的参与,这是一个微管细胞皮质相互作用调解器.
- 利用计算模拟来模型划分平面切换.
主要成果:
- PLETHORA转录因子激活了辅酶信号,导致了MAP65蛋白的上调.
- MAP65上调调节通过CLASP重新定位皮质微管阵列.
- CLASP的局部化取决于微管和MAP65.5.
- 模拟显示了自我组织的微管体特性和CLASP偏差驱动90度分割平面开关.
结论:
- 转录因子介导的辅酶信号激活控制了植物细胞分裂的方向.
- MAP65-CLASP-微管道是调节形成细胞分裂平面的关键.
- 这项研究揭示了从转录因子到面向细胞分裂的细胞机械的调节级联.
相关概念视频
Determining the Plane of Cell Division
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function.
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Determining the Plane of Cell Division
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function.
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Cell Motility through Blebbing
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
Molecular Factors Affecting Cell Division
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
The Phragmoplast
Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
The...
The...
The Contractile Ring
Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...

