保存的C. elegans基因的非激活会激活病原体和异生菌相关的防御系统
1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Cell
|April 17, 2012
概括
破坏C. elegans中必不可少的细胞功能会触发食物细菌的避开,并激活免疫反应. 这表明监控系统通过监测细胞核心活动来检测病原体.
科学领域:
- 尼马生物学的生物学
- 细胞生物学 细胞生物学
- 神经内分泌学神经内分泌学
背景情况:
- 线虫Caenorhabditis elegans通常会避开病原体和毒素,而被营养细菌吸引.
- 核心细胞活动,如翻译,呼吸和蛋白质循环,对于生物的功能至关重要.
研究的目的:
- 研究核心细胞活动的破坏如何影响C. elegans的行为和免疫反应.
- 确定参与调解这些行为和生理变化的信号通路.
主要方法:
- RNA干扰 (RNAi) 破坏基因功能和细胞核心过程.
- 毒素介导的细胞活动的破坏.
- 行为测试来测量对细菌的吸引力/厌恶.
- 基因表达分析以评估排毒和免疫效应因子激活.
主要成果:
- 通过RNAi或毒素诱导的核心细胞活动 (翻译,呼吸,蛋白质周转) 受到干扰,诱发了对有吸引力的细菌的行为回避.
- 这些干扰也导致了排毒和先天性免疫因子的表达,即使没有外部毒素或病原体.
- 非神经元组织的干扰足以引发厌恶,表明神经内分泌控制机制.
- 这种厌恶行为需要血清和Jnk激酶信号通路.
结论:
- 细胞监测途径监测细胞核心活动,以检测病原体及其毒性因素.
- 一个神经内分泌轴,涉及血清和Jnk信号传递,调解行为和免疫对细胞压力的反应.
- 这些监视和内分泌通路的变化可能解释了对食物,药物和微生物的不同反应.
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