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相关概念视频

Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...

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相关实验视频

Updated: May 30, 2026

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

携带子β-环球蛋白基因编码序列的SV40重组剂

D H Hamer, K D Smith, S H Boyer

    Cell
    |July 1, 1979
    PubMed
    概括
    此摘要是机器生成的。

    创建了SV40重组剂来研究基因表达. 这些病毒表明,一个特定的SV40段,可能是拼接区域,对于产生稳定的信使RNA和新蛋白质至关重要.

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    Last Updated: May 30, 2026

    Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
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    Published on: April 4, 2019

    Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
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    科学领域:

    • 分子生物学分子生物学
    • 病毒学 病毒学
    • 基因工程是一种基因工程.

    背景情况:

    • 猿类病毒40 (SV40) 是研究真核生物基因调节的一个有价值的工具.
    • 了解病毒调节序列对于基因治疗和生物技术至关重要.

    研究的目的:

    • 研究SV40调节序列在基因表达中的作用.
    • 评估SV40重组剂在产生混合转录和新基因产品方面的实用性.

    主要方法:

    • 构建和传播两个SV40重组病毒.
    • 用子β-环球蛋白编码序列替换SV40晚期基因区域的部分.
    • 使用放射性免疫试验分析病毒转录稳定性和蛋白质合成.

    主要成果:

    • 一种SV40复合物产生了稳定的SV40-蛋白混合转录,该转录在受感染的子细胞中转化为可检测的β-蛋白.
    • 缺少RNA剪接区域的第二个重组体未能产生稳定的全球因子转录或可检测的β-全球因子.
    • 这些发现确定了一个500bp的SV40段,可能是拼接区域,对于稳定的mRNA积累至关重要.

    结论:

    • SV40拼接区域对于稳定的病毒晚期mRNA的积累至关重要.
    • 混合转导病毒作为有效的工具来表征病毒调节元件.
    • 可以利用SV40重组剂在培养细胞中合成新基因产品.