在盐水Artemia中的聚合物环球蛋白的演变
A M Manning1, C N Trotman, W P Tate
1Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Nature
|December 13, 1990
概括
像盐水这样的无脊椎动物具有由化环球蛋白基因形成的聚合血红蛋白. 分析显示,这些基因编码了多个功能域,这表明血红蛋白进化中的古老基因融合事件.
科学领域:
- 生物化学 生物化学
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 无脊椎动物表现出多样化的血红蛋白结构,包括聚合物形式,每种多基具有多个血红蛋白结合域.
- 这些聚合血红素可能来自于祖先的单质环球蛋白基因的融合.
研究的目的:
- 为了分析来自盐水Artemia的聚合环球蛋白基因的转录.
- 为了阐明Artemia的聚合血红蛋白的结构和进化起源.
主要方法:
- 对辅助DNA (cDNA) 克隆编码Artemia二元血红蛋白子单元的分析.
- 衍生氨基酸序列的生物信息分析,以预测蛋白质结构和进化史.
主要成果:
- 阿尔特米亚的环球蛋白信使RNA编码了九个类似于肌球蛋白的域,由特定的序列连接在一起.
- 每个域都含有保存的血结合残留物,预计将采用类似于肌球蛋白的折叠.
- 序列分析表明,这些域是重复的单体,大约2亿年前融合在一起.
结论:
- 在Artemia中的聚合环球蛋白基因是通过融合多个祖先的环球蛋白单元而演变的.
- 基因融合是无脊椎动物中聚合血红蛋白出现的可能机制.
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