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基本状态多能性的转录和表观基因组基础
Hendrik Marks1, Tüzer Kalkan, Roberta Menafra
1Department of Molecular Biology, Faculty of Science, Radboud University, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
Cell
|May 1, 2012
概括
在血清中的小鼠胚胎干细胞 (ESC) 显示出比2i介质中的更多的变异. 2i条件被认为是一个天真的基本状态,它揭示了不同的转录和表观遗传特征.
科学领域:
- 发育生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在血清中培养的胚胎干细胞 (ESC) 显示异质性.
- 建议2i介质 (Mek和GSK3抑制剂) 诱导ESCs的天真基底状态.
- 不同的分子形状可能是ESC国家的差异的基础.
研究的目的:
- 为了比较在血清中培养的小鼠ESCs的转录组和表观组概况,与2i介质相比.
- 调查由2i.诱导的拟议的天真基底状态的特征.
- 了解不同ESC培养条件下基因表达的调控机制.
主要方法:
- 转录组概况 (RNA测序) 的ESCs.
- 表观基因组分析,包括基因组修饰分析 (例如,H3K27me3) 和染色质可访问性.
- 评估基因表达模式和染色质状态.
- 功能性测试用于评估差异化潜力.
主要成果:
- 血清和2i培养的ESC表现出明显的转录组和表观基因组配置文件.
- 2i处理的ESCs表现出减少的基因表达和更少的压制性组织蛋白标记 (H3K27me3) 和双价域.
- 尽管存在表观遗传差异,但两种培养条件都产生了具有相似差异化潜力的ESC.
- RNA聚合酶II暂停抑制2i条件ESC中早期发育基因转录.
结论:
- 天真基底状态的特点是转录潜能和允许的染色体.
- 从基本状态的退出可能不需要转移稳定的中间或多线程初始化.
- 了解ESC状态对于发育生物学和再生医学研究至关重要.
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